The aim of this study was to develop and validate a fast and simple reversed-phase HPLC\nmethod for simultaneous determination of four cardiovascular agentsââ?¬â?atorvastatin, simvastatin,\ntelmisartan and irbesartan in bulk drugs and tablet oral dosage forms. The chromatographic\nseparation was accomplished by using Symmetry C18 column (75 mm Ã?â?? 4.6 mm; 3.5 Ã?¼) with a\nmobile phase consisting of ammonium acetate buffer (10 mM; pH 4.0) and acetonitrile in a ratio\n40:60 v/v. Flow rate was maintained at 1 mL/min up to 3.5 min, and then suddenly changed to\n2 mL/min till the end of the run (7.5 min). The data was acquired using ultraviolet detector monitored\nat 220 nm. The method was validated for linearity, precision, accuracy and specificity. The developed\nmethod has shown excellent linearity (R2 > 0.999) over the concentration range of 1ââ?¬â??16 Ã?¼g/mL.\nThe limits of detection (LODs) and limits of quantification (LOQs) were in the range of 0.189ââ?¬â??0.190\nand 0.603ââ?¬â??0.630 Ã?¼g/mL, respectively. Inter-day and intra-day accuracy and precision data were\nrecorded in the acceptable limits. The new method has successfully been applied for quantification of\nall four drugs in their tablet dosage forms with percent recovery within 100 Ã?± 2%.
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